pie-1 wormbase gene report
NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.
23 Slice4D movies
pie-1%PHplcd%GFP_0aj_slc.mov
pie-1%PHplcd%GFP_0ak_slc.mov
pie-1%PHplcd%GFP_0ax_slc.mov
pie-1%PHplcd%GFP_0ay_slc.mov
pie-1%PHplcd%GFP_0bk_slc.mov
pie-1%PHplcd%GFP_1aj_slc.mov
pie-1%PHplcd%GFP_1ak_slc.mov
pie-1%PHplcd%GFP_1ax_slc.mov
pie-1%PHplcd%GFP_1bk_slc.mov
pie-1%PHplcd%GFP_2aj_slc.mov
pie-1%PHplcd%GFP_2ak_slc.mov
pie-1%PHplcd%GFP_2bk_slc.mov
pie-1%PHplcd%GFP_3bk_slc.mov
pie-1%PHplcd%GFP_4bk_slc.mov
pie-1%PHplcd%GFP_2E1_slc.mov
pie-1%PHplcd%GFP_2E3_slc.mov
pie-1%PHplcd%GFP_2E5_slc.mov
pie-1%PHplcd%GFP_2E7_slc.mov
pie-1%PHplcd%GFP_2E9_slc.mov
pie-1%PHplcd%GFP_E1_slc.mov
pie-1%PHplcd%GFP_E3_slc.mov
pie-1%PHplcd%GFP_E5_slc.mov
pie-1%PHplcd%GFP_E7_slc.mov
32 Stereo4D movies (23 embryos)
pie-1%PHplcd%GFP_0aj_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_0ak_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_0ax_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_0ay_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_0bk_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_1aj_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_1ak_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_1ax_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_1bk_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2aj_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2ak_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2bk_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_3bk_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_4bk_pryx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E1_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E1_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E3_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E3_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E5_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E5_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E7_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E7_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E9_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_2E9_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_E1_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_E1_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_E3_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_E3_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_E5_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_E5_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_E7_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%PHplcd%GFP_E7_pry.mov (also viewable in ®© RedCyanStereo)
2 comments:
Movies are made from hanging embryo mounts (random orientation for each embryo).
Expression is high at earliest recorded timepoints. Expression fading away from peak by the time embryonic movement occurs. High expression is retained in germ-cell precursors.
GFP is localized to the inner plasma membrane by the pleckstrin-homology domain of phospholipase C-delta. Outlines of all cells in the embryo are distinct.
0Jaj = Starts at 12-cell stage, continues 190 minutes (though gastrulation.
0Jax = Ventral view. Starts just after 24-cell stage, runs through most of gastrulation, elongation, movement.
0Jay = Dorsolateral view, 1 min/timestep. Absolute developmental time is mislabeled. Covers from comma to 2-fold stage.
0Jbk = Dorsal view. Starts shortly before morphogenesis, carries through elongation and movement.
1Jaj = Starts at 12-cell stage. Proceeds through some of gastrulation (190 minutes total).
1Jax = Ventral view. Beautiful ingression of Ea+ Ep during first movements of gastrulation, conhineves through rest of gastrulation and embryonic elongation and movement.
1Jbk = Lateral view. Begins in mid-gastrulation and continues through morphogenesis and movement.
2Jaj = Begins at 8-cell stage and continues 190 minutes through much of cleavage and gastrulation.
2Jbk = Dorsolateral view. Begins in mid-gastrulation and continues through morphogenesis and movement.
3Jbk = Lateral view. Begins in mid-gastrulation and continues through morphogenesis and movement.
4Jbk = ventrolateral view. Begins in mid-gastrulation and continues through morphogenesis and movement.
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